Marlene Grenon1, Christopher D. Owens1, Hugh Alley1, Karen Chong1, Eric Vittinghoff1, William Harris2, Millie Hughes-Fulford1, Joseph Rapp1, Michael Conte1
1UCSF, San Francisco, CA; 2University of South Dakota, Vermillion, SD.
OBJECTIVES: Dietary intake of n-3 PUFAs has been associated with cardiovascular disease, but the relationship to PAD is unclear. PAD patients have an increased burden of systemic inflammation. In a cross-sectional cohort study, we evaluated the relationship between n-3 PUFAs content of red blood cells (omega-3 index) and biomarkers of inflammation.
METHODS: This was a prospective cohort study of patients (n=83) presenting to vascular surgery clinic for evaluation of PAD. We used linear regression to evaluate the independent association between the omega-3 index (gas chromatography) and inflammation (hsCRP, IL-6, TNF-α and ICAM-1; ELISA kits).
RESULTS: Seventy patients had PAD, while 13 were found to have a normal ankle-brachial index (ABI). Mean (±SD) age was 67±7 years. Mean ABI was 0.85±0.23. The omega-3 index decreased across AHA hsCRP categories (Figure 1, p=0.04). One percentage point decrease in the omega-3 index was associated with increases in CRP (14%, 95% CI 0, 25, p=0.04), IL6 (8%, 95% CI 1, 15, p=0.02) and possibly ICAM-1 (4%, 95% CI -1, 12, p=0.13), but not TNF-α. After adjusting for age, race, HDL, smoking status, ABI and the body-mass index, the omega-3 index remained significantly (negatively) associated with systemic inflammation, as measured by hsCRP in a male population at risk or suffering from PAD (p=0.05).
CONCLUSIONS: In a contemporary cohort of patients with PAD, the omega-3 index was negatively associated with biomarkers of inflammation. Our findings suggest a rationale for future studies of dietary manipulation of omega-3 index to reduce inflammation in patients with PAD.
AUTHOR DISCLOSURES: H. Alley: Nothing to disclose; K. Chong: Nothing to disclose; M. Conte: Nothing to disclose; M. Grenon: Nothing to disclose; W. Harris: Nothing to disclose; M. Hughes-Fulford: Nothing to disclose; C. D. Owens: Nothing to disclose; J. Rapp: Nothing to disclose; E. Vittinghoff: Nothing to disclose.
Posted April 2013